culture medium (Beyotime)

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Beyotime culture medium
Culture Medium, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum+free+culture+medium/293+Serum-free+Culture+Medium/pmc12854987-88-5-22
Average 99 stars, based on 78 article reviews

culture medium - by Bioz Stars, 2026-10

99/100 stars

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Staining:

Article Title: NCOA4-mediated ferroptosis conducive to EMT inhibition in colorectal cancer cells was achieved through the PI3K/AKT/mTOR pathway
Article Snippet: .. Finally, the cells were stained with H 2 DCF-DA in serum-free culture medium and incubated for 30 min (Active Oxygen Detection Kit, Beyotime Biotechnology). .. The intracellular ROS concentration was assayed via a flow cytometer (Cytoflex, Beckman Coulter, USA).

Article Title: NCOA4-mediated ferroptosis conducive to EMT inhibition in colorectal cancer cells was achieved through the PI3K/AKT/mTOR pathway.
Article Snippet: .. Finally, the cells were stained with H2DCF-DA in serum-free culture medium and incubated for 30 min (Active Oxygen Detection Kit, Beyotime Biotechnology). .. The intracellular ROS concentration was assayed via a flow cytometer (Cytoflex, Beckman Coulter, USA).

Incubation:

Article Title: RHOT1-mediated molecular mechanism of mitochondrial dysfunction and its phenotypic effects on gastric cancer cells
Article Snippet: .. The treatment group was directly treated with 10 μ M DCFH-DA dissolved in serum-free culture medium (1 ml) and incubated at 37°C for 30 min. Fluorescence was detected by flow cytometry at an excitation wavelength of 488 nm and an emission wavelength of 530 nm and changes in MMP were detected using the Mitochondrial Membrane Potential Detection Kit (Beyotime Institute of Biotechnology). ..

Article Title: NF-κB activation as a pro-survival signal from pharmacological inhibition of pyruvate dehydrogenase kinase 1 in non-small-cell lung carcinoma cell models
Article Snippet: .. The cells were then treated with various compounds or 0.1% DMSO for 4 h. After the incubation, the cells were washed three times with serum-free culture medium and incubated with 1 mL of 10 μM DCFH-DA (Beyotime, Shanghai, China) diluted in serum-free medium at 37°C for 20 min. .. Finally, the cells were washed three times with serum-free medium and imaged using the Invitrogen EVOS FL Auto Cell Imaging System (ThermoFisher Scientific, Waltham, MA, USA) for cell imaging.

Article Title: NF-κB activation as a pro-survival signal from pharmacological inhibition of pyruvate dehydrogenase kinase 1 in non-small-cell lung carcinoma cell models.
Article Snippet: .. The cells were then treated with various compounds or 0.1% DMSO for 4 h. After the incubation, the cells were washed three times with serum-free culture medium and incubated with 1 mL of 10 μM DCFH-DA (Beyotime, Shanghai, China) diluted in serum-free medium at 37◦C for 20 min. .. Finally, the cells were washed three times with serum-free medium and imaged using the Invitrogen EVOS FL Auto Cell Imaging System (ThermoFisher Scientific, Waltham, MA, USA) for cell imaging.

Article Title: RHOT1‑mediated molecular mechanism of mitochondrial dysfunction and its phenotypic effects on gastric cancer cells.
Article Snippet: .. The treatment group was directly treated with 10 μM DCFH‐DA dissolved in serum‐free culture medium (1 ml) and incubated at 37 ̊C for 30 min. Fluorescence was detected by flow cytometry at an excitation wavelength of 488 nm and an emission wavelength of 530 nm and changes in MMP were detected using the Mitochondrial Membrane Potential Detection Kit (Beyotime Institute of Biotechnology). ..

Fluorescence:

Flow Cytometry:

Membrane:

Small Interfering RNA:

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice
Article Snippet: .. The TSG-6 small interfering RNA (siRNA)/short hairpin RNA (shRNA) was then carefully added to 250 μL of serum-free culture medium and mixed thoroughly with 5 μL of LipofectamineTM 3000 (Cat.No.C0533, Beyotime, Shanghai, China) and 250 μL of serum-free medium. ..

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culture medium (Beyotime)

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Flow Cytometry:

Membrane:

Small Interfering RNA:

shRNA:

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